Response to BMP4 signalling during ES cell differentiation defines intermediates of the ectoderm lineage

The formation and differentiation of multipotent precursors underlies the generation of cell diversity during mammalian development. Recognition and analysis of these transient cell populations has been hampered by technical difficulties in accessing them in vivo. In vitro model systems, based on the differentiation of embryonic stem (ES) cells, provide an alternative means of identifying and characterizing these populations. Using a previously established mouse ES-cell-based system that recapitulates the development of the ectoderm lineage we have identified a transient population that is consistent with definitive ectoderm. This previously unidentified progenitor occurs as a temporally discrete population during ES cell differentiation, and differs from the preceding and succeeding populations in gene expression and differentiation potential, with the unique ability to form surface ectoderm in response to BMP4 signalling.Nathan T. Harvey, James N. Hughes, Ana Lonic, Charlotte Yap, Catherine Long, Peter D. Rathjen and Joy Rathje

Around the Table: Studies in Co-located Collaboration

Abstract. This video illustrates how pervasive technology can be designed to support more effectively collaborative working. To begin, it shows what happens when small co-located groups try to work together using a single PC or whiteboard: it can be frustrating and encourage one person to dominate. The video then explains our ‘multiple entry points ’ framework, intended to inform the design of shared workspaces, that can facilitate numerous and natural ways of taking control and contributing to the ongoing work by all group members. Two set-ups are presented with increasing number of entry points; (i) a shared touch screen tabletop and (ii) a large physical-digital space comprising a tabletop interlinked with tagged physical objects. The findings from two user studies are outlined; the tabletop was found to facilitate more collaborative decisionmaking for a simple design task while the extended version, with a larger number of entry points, promoted more fluid and diverse collaborative interactions. Furthermore, it was found that the quiet group members participated considerably more in the physical design activities.

Silicone Applicator Cleaning Improvement

Silicone is curing inside the applicator tips due to process not being standardized. Due to silicone curing inside the tips, the employees are having to repeat the cleaning process resulting in added labor expenses.</p

The family cook book /

Advertisements throughout text."Recommendations from a Journal of Health": p. 90-91.Mode of access: Internet.Burgundy cloth binding; gilt cover title.ACQ: 36549-A; Bruce J. Ramer; 0010000; 5/18/2005

Silicone Applicator Cleaning Improvement

Cardinal Glass is a residential glass manufacturer for doors and windows. Cardinal glass was first founded in 1962 by a man named M.L. Gordon. Today, they employ 6,000 employees across 37 manufacturing plants throughout the United States. The Greenfield, Iowa, plant distributes to local large window manufacturers. Cardinal Glass is separated into five divisions. They are as followed:FG- Float & High Volume Tempered Glass; CT- Custom Tempered Glass; LG- Laminated Glass; CG- coated glass and optical mirrors; and IG- Insulating Glass. The Client is experiencing problems with silicone curing in applicator tips due to lack of a better process. The silicone cured tips are causing re-work that is costing the company thousands of dollars in labor.</p